Upload NowIn this article we will discuss about the principle, requirements and procedure for Agarose gel electrophoresis. Agarose gel electrophoresis is a simple and highly effective method for separating, identifying and purifying DNA fragments. Separation is carried out under an electric field applied to gel matrix. Because of the negatively charged phosphates along the backbone, DNA […]
Agarose Gel Electrophoresis | Molecular Biology
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Enzyme-Immunoassay (EIA): Principle and Procedure | Medicine
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EIA technique is generally used in medicine to detect the antigen or antibodies in serum samples and also in immunodiagnosis of several infectious diseases. It is used for semiquantitative determination of the concentration of certain antigens/antibodies. The ELISA technique was first introduced in early 1970s by Engvall and Perlmann. Principle: The antibodies against the antigen […]
Separating Micro-Weights of Proteins: PAGE | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Polyacrylamide Gel Electrophoresis (PAGE). Micro-weights of proteins can be separated and characterised rapidly and relatively sensitive, using PAGE. Buffer gels (non-denaturing) as well as Sodium Dodecyl Sulphate (SDS) containing denaturing gels are used. Separation in buffer gels depends on both the charge […]