Tag Archives | Southern Blotting

In this article we will discuss about the technique of southern blotting for transfer of DNA from gels to nitrocellulose. This technique was first described by E.M. Southern in 1975. It is useful for analysis of Restriction Fragment Length Polymor­phisms (RFLPs). Using gel electrophoresis in combination with restriction endonuclease analysis, a detailed and accurate physical […]