Upload NowIn this article we will discuss about the isolation of RNA with its protocols. Learn how to isolate:- 1. Total RNA from Bacterial Cells 2. Total RNA from Plant Tissues 3. Hot Phenol of RNA from Plant Tissues 4. Messenger RNA. 1. How to Isolate Total RNA from Bacterial Cells? Protocol: As usual when working […]
How to Isolate RNA: Bacterial Cells, Plant Tissues and Messenger RNA
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Isolation of RNA | Molecular Biology
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In this article we will discuss about the requirements and procedure for isolation of RNA using Phenol-SDS method. SDS dissociates the ribonucleoprotein complex into RNA and protein, deproteinised by phenol and the free RNA left in aqueous solution is precipitated in cold after adding alcohol. Requirements: Reagents: 1. Extraction buffer-pH 9.0: Tris HCl (0.1 M): […]
Estimation of RNA | Biotechnology
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In this article we will discuss about the principle, requirements and procedure for estimation of RNA. Principle: Ribose (a pentose) in presence of hot acid is converted to furfural which then reacts with orcinol to yield a green colour, read at 660 nm. Requirements: 1. Orcinol: Prepare 10% (W/V) FeCl3. 6H20 solution. To 0.5 ml […]