In this article we will discuss about the principle, requirements and procedure for Polyacrylamide Gel Electrophoresis (PAGE). Micro-weights of proteins can be separated and characterised rapidly and relatively sensitive, using PAGE. Buffer gels (non-denaturing) as well as Sodium Dodecyl Sulphate (SDS) containing denaturing gels are used. Separation in buffer gels depends on both the charge […]
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