Upload NowIn this article we will discuss about the principle, requirements and procedure for Polyacrylamide Gel Electrophoresis (PAGE). Micro-weights of proteins can be separated and characterised rapidly and relatively sensitive, using PAGE. Buffer gels (non-denaturing) as well as Sodium Dodecyl Sulphate (SDS) containing denaturing gels are used. Separation in buffer gels depends on both the charge […]
Separating Micro-Weights of Proteins: PAGE | Molecular Biology
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Northern Blotting and Hybridisation | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for northern blotting and hybridisation. By this method specific mRNA sequences in RNA preparations can be detected. Northern blots are carried out in order to detect as to which of the several RNA molecules on agarose gel hybridize to a particular probe. It […]
Polymerase Chain Reaction (PCR) | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Polymerase Chain Reaction (PCR). PCR is a simple method for in vitro enzymatic amplification of specific nucleic acids using multiple cycles of template denaturation, primer annealing and primer elongation. From as little as a single piece of target DNA, enough copies can […]