Upload NowIn this article we will discuss about the principle, requirements and procedure for Random Amplified Polymorphic DNA (RAPD) analysis. Randomly amplified polymorphic DNA (RAPD) was developed by William et al. (1990) to detect genetic polymorphism in crop plants. Polymerase chain reaction (PCR) is an in vitro method of nucleic acid synthesis by which a particular […]
Random Amplified Polymorphic DNA (RAPD) Analysis | Molecular Biology
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Agarose Gel Electrophoresis | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Agarose gel electrophoresis. Agarose gel electrophoresis is a simple and highly effective method for separating, identifying and purifying DNA fragments. Separation is carried out under an electric field applied to gel matrix. Because of the negatively charged phosphates along the backbone, DNA […]
Amplified Fragment Length Polymorphism (AFLP) Analysis | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Amplified Fragment Length Polymorphism (AFLP) analysis. Amplified Fragment Length Polymorphism is a nucleic acid finger printing method to find out genetic variation that exists between closely related genes. This method was designed by Zabeau and Vos (1993) and Vos et al. (1995). […]