Upload NowIn this article we will discuss about the principle, requirements and procedure for Agarose gel electrophoresis. Agarose gel electrophoresis is a simple and highly effective method for separating, identifying and purifying DNA fragments. Separation is carried out under an electric field applied to gel matrix. Because of the negatively charged phosphates along the backbone, DNA […]
Agarose Gel Electrophoresis | Molecular Biology
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Amplified Fragment Length Polymorphism (AFLP) Analysis | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Amplified Fragment Length Polymorphism (AFLP) analysis. Amplified Fragment Length Polymorphism is a nucleic acid finger printing method to find out genetic variation that exists between closely related genes. This method was designed by Zabeau and Vos (1993) and Vos et al. (1995). […]
Silver Staining of Proteins | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for silver staining of proteins. Polypeptides even upto 0.1 µg present on gel can be visualised by staining with amido black 10B dye or Coomassie brilliant blue R250. Silver staining is useful since some proteins occur in very small quantities and are difficult […]