Upload NowIn this article we will discuss about the restriction digestion of DNA and DNA samples. Restriction Digestion of DNA: Restriction endonucleases cut DNA molecules into smaller pieces. They cut DNA at the specific recognition sequence, so that it is no longer susceptible to cleavage by the endonucleases. These enzymes are present in vivo in bacteria […]
Restriction Digestion of DNA and DNA Samples | Molecular Biology
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Separating Micro-Weights of Proteins: PAGE | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for Polyacrylamide Gel Electrophoresis (PAGE). Micro-weights of proteins can be separated and characterised rapidly and relatively sensitive, using PAGE. Buffer gels (non-denaturing) as well as Sodium Dodecyl Sulphate (SDS) containing denaturing gels are used. Separation in buffer gels depends on both the charge […]
Northern Blotting and Hybridisation | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for northern blotting and hybridisation. By this method specific mRNA sequences in RNA preparations can be detected. Northern blots are carried out in order to detect as to which of the several RNA molecules on agarose gel hybridize to a particular probe. It […]