Upload NowHybridisation reactions are based on the ability of denatured DNA to anneal to a complementary strand present in a nearby environment at a temperature below that of theirs. The two important features of hybridisation are: (1) These reactions are specific i.e. the probe will only bind to targets with complementary sequence. (2) These reactions will […]
Southern Hybridisation | Molecular Biology
Article shared by : 
Isolation of Bacterial Genomic DNA | Molecular Biology
Article shared by :
In this article we will discuss about the principle, requirements and procedure for isolation of bacterial genomic DNA. Bacterial chromosomes are single supercoiled double stranded circular DNA molecules. Plasmid DNA has a size of 0.1 to 0.5% of the chromosome. During plasmid purification preferential recovery of circular plasmid DNA over linear chromosomal (genomic) DNA is […]
Isolation of Plasmid DNA | Molecular Biology
Article shared by :
In this article we will discuss about the principle, requirements and procedure for isolation of plasmid DNA using alkaline lysis method. Principle: The alkaline lysis method of plasmid DNA is based on lysis of bacteria by treating it with SDS and NaOH, neutralisation of this mixture with potassium acetate which causes rapid annealing of the […]