Upload NowThe following points highlight the two methods used for extraction of plant genomic DNA. The methods are: 1. CTAB Method 2. Potassium Acetate Method. 1. CTAB Method: Plant genomic DNA is required mainly for mapping and isolation of genes for genetic engineering. If the protocol is good, pure and intact DNA can be obtained. The […]
Extraction of Plant Genomic DNA | Molecular Biology
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Southern Hybridisation | Molecular Biology
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Hybridisation reactions are based on the ability of denatured DNA to anneal to a complementary strand present in a nearby environment at a temperature below that of theirs. The two important features of hybridisation are: (1) These reactions are specific i.e. the probe will only bind to targets with complementary sequence. (2) These reactions will […]
Isolation of Bacterial Genomic DNA | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for isolation of bacterial genomic DNA. Bacterial chromosomes are single supercoiled double stranded circular DNA molecules. Plasmid DNA has a size of 0.1 to 0.5% of the chromosome. During plasmid purification preferential recovery of circular plasmid DNA over linear chromosomal (genomic) DNA is […]