Upload NowIn this article we will discuss about the rules, precautions and basic requirements of a laboratory. Laboratory Rules and Precautions: A new worker in a laboratory must have an orientation before starting the work. The new worker should be given instructions as to how to operate major equipment including microscope, analytical balance and pH meter. […]
Laboratory: Rules and Basic Requirements | Biotechnology
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Transformation of Bacterial Gene | Molecular Biology
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This was the first type of transfer of genetic information discovered in bacteria. The first transformation was done by Fredrick Griffith in 1928. In this method a foreign plasmid DNA is introduced into a bacterium and uses this to multiply plasmids in order to obtain large amounts of it. Principle: The E. coli (DH5α) used […]
Immunoadsorption: Principle and Procedure | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for immunoadsorption. Principle: Antigen-antibody complexes are formed when transferred proteins on nitrocellulose paper are allowed to react with diluted antis- era. This antigen-antibody complex bound to the nitrocellulose membrane are allowed to react with alkaline phosphatase conjugated anti-rabbit-IgG. The colour develops blue to […]