Upload NowIn this article we will discuss about the principle, requirements and procedure for isolation of coliphages from sewage. Principle: Samples like raw sewage when inoculated with a susceptible host on an agar medium will form plaques of the phage. There are various steps involved in isolation of phages: (i) Collection of phage containing samples from […]
Isolation of Coliphages from Sewage | Microbial Biotechnology
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Litmus Milk Reactions: Requirements and Procedure | Bacteria
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After reading this article you will learn about the requirements and procedure of litmus milk reactions. Milk sugar lactose, the milk proteins casein, lacto-albumin and lacto-globulin are the milk substrates that can be transformed by microorganisms, bringing about changes in pH. In order to distinguish the metabolic changes, a pH indicator, the oxidation reduction indicator […]
Cultivation of Anaerobic Organisms | Microbial Biotechnology
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The following points highlight the top two methods used for cultivation of anaerobic organisms. The methods are: 1. Shake Culture Method 2. Pyrogallic Acid: NaOH Method. 1. Shake Culture Method: Principle: The anaerobic organism does not require molecular oxygen for growth and hence will grow within the agar medium and E. coli above the medium […]