Upload NowThe following points highlight the top eight methods of sterilization. The methods are: 1. Boiling 2. Intermittent Heating 3. Autoclaving 4. Dry Heat Sterilisation Indirect Heat 5. Open Flame Sterilisation 6. Filtration 7. Chemical Sterilisation 8. Pasteurisation. Method # 1. Boiling: In the absence of heat resistant spores, boiling will free liquids of living cells. […]
Methods of Sterilisation | Laboratory | Biotechnology
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Preparation of Buffers and Solutions | Laboratory | Biotechnology
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The following guidelines should be followed while making reagents, buffers, etc. 1. Use highest grade of reagents wherever possible. 2. Prepare all solutions with double distilled or distilled water. 3. Autoclave solutions whenever possible. Solutions that cannot withstand autoclaving should be sterilized using 0.2 mm filter. 4. pH meter should carefully be checked using freshly […]
Determination of pKa Values using pH Meter | Biotechnology
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In this article we will discuss about the determination of pKa values using pH meter. Titration between a Strong Acid and a Strong Base: Requirements: 1. pH meter. 2. 0.1(M) HCl (Normality of HCl is ll.5). 3. 0.1 (M) NaOH (dilute from 1(M) stock solution). 4. Standard buffer 0.05(M) potassium hydrogen phthalate, pH 4.01. 5. […]