Upload NowThe following guidelines should be followed while making reagents, buffers, etc. 1. Use highest grade of reagents wherever possible. 2. Prepare all solutions with double distilled or distilled water. 3. Autoclave solutions whenever possible. Solutions that cannot withstand autoclaving should be sterilized using 0.2 mm filter. 4. pH meter should carefully be checked using freshly […]
Preparation of Buffers and Solutions | Laboratory | Biotechnology
Article shared by : 
Preparation of Buffers and Solutions | Laboratory | Biotechnology
Article shared by :
The following guidelines should be followed while making reagents, buffers, etc. 1. Use highest grade of reagents wherever possible. 2. Prepare all solutions with double distilled or distilled water. 3. Autoclave solutions whenever possible. Solutions that cannot withstand autoclaving should be sterilized using 0.2 mm filter. 4. pH meter should carefully be checked using freshly […]
Determination of pKa Values using pH Meter | Biotechnology
Article shared by :
In this article we will discuss about the determination of pKa values using pH meter. Titration between a Strong Acid and a Strong Base: Requirements: 1. pH meter. 2. 0.1(M) HCl (Normality of HCl is ll.5). 3. 0.1 (M) NaOH (dilute from 1(M) stock solution). 4. Standard buffer 0.05(M) potassium hydrogen phthalate, pH 4.01. 5. […]