Upload NowIn this article we will discuss about the principle, requirements and procedure for Amplified Fragment Length Polymorphism (AFLP) analysis. Amplified Fragment Length Polymorphism is a nucleic acid finger printing method to find out genetic variation that exists between closely related genes. This method was designed by Zabeau and Vos (1993) and Vos et al. (1995). […]
Amplified Fragment Length Polymorphism (AFLP) Analysis | Molecular Biology
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Restriction Digestion of DNA and DNA Samples | Molecular Biology
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In this article we will discuss about the restriction digestion of DNA and DNA samples. Restriction Digestion of DNA: Restriction endonucleases cut DNA molecules into smaller pieces. They cut DNA at the specific recognition sequence, so that it is no longer susceptible to cleavage by the endonucleases. These enzymes are present in vivo in bacteria […]
Purification of DNA | Molecular Biology
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In this article we will discuss about the principle, requirements and procedure for purification of DNA. Principle: DNA is removed from gel by dissolving the gel in cesium chloride and DNA is taken out with ethidium bromide in T.E. buffer, centrifuged and DNA is removed. Ethidium bromide is extracted with n-butanol, and isopropanol precipitates out […]