In this article we will discuss about the principle, requirements and procedure for estimation of leghaemoglobin. Kubo (1939) recognised the oxygen-binding haemoprotein in the root nodules of leguminous plants. Hence pan of the Leghaemoglobin is synthesised by the bacterium (Rhizobium) and the apoprotein (globin) is coded by plant DNA Oxygen is diffused by Lb across […]
Estimation of Leghaemoglobin | Leguminous Plants | Biotechnology
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Estimation and Micro Estimation of Citric Acid | Biotechnology
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In this article we will discuss about the estimation and micro estimation of citric acid. Estimation of Citric Acid: Titrable Acidity Method: Requirements: 1. Fermented broth. 2. 0.1 (N) NaOH. 3. 1.0(N) Succinic acid. 4. Phenolphthalin. 5. Titration set. Procedure: 1. Standardise NaOH using 1(N) succinic acid. 2. Take in two separate 100 ml conical […]
Isolation of Plant Pathogens | Biotechnology
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In this article we will discuss about the principle, requirements and procedure for isolation of plant pathogens. Principle: A medium with a carbon source and inorganic salts will permit the growth of plant pathogens which can be maintained on agar slants of the same medium. Requirements: 1. Infected plant parts, leaves/stem infected with Alternaria. 2. […]