The following points highlight the top two techniques used for screening of antibiotic producing organisms. The techniques are: 1. Crowded Plate Technique 2. Determination of Antimicrobial Spectrum Giant Colony Technique. 1. Crowded Plate Technique: Principle: When soil or suspension with lower dilutions is inoculated, large number of microorganisms grow on the agar plate. Those which […]
Screening of Antibiotic Producing Organisms | Biotechnology
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Rhizobium: Isolation and Authentication | Microbial Biotechnology
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In this article we will discuss about the isolation and authentication of rhizobium. Isolation of Rhizobium: Rhizobium is a symbiotic N2 fixer found to occur as bacteroids in the root nodules of leguminous plants. They can be easily isolated and cultured in vitro. Rhizobia are Gram- negative rods which are motile with bi-polar, sub-polar and […]
Observation of Fungi and Actinomycetes | Biotechnology
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The following points highlight the two methods used for observation of fungi and actinomycetes. The methods are: 1. Slide Culture 2. Cover Slip Culture. Method # 1. Slide Culture: Principle: This method is used for fungi and actinomycetes to observe the conidiophores, the conidiogenous cells, their mode of conidial formation (conidial ontogeny) and conidia under […]