In this article we will discuss about the gene isolation and cloning of DNA. The formation of new combinations of genetic material by the insertion of nucleic acid produced outside the cell into a virus, bacterial plasmid or any other vector system to allow its incorporation into a host organism in which it is capable […]
Gene Isolation and Cloning of DNA | Biotechnology
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Isolation of Animal Tissue Culture | Biotechnology
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In this article we will discuss about the isolation of animal tissue culture. Before culture, animal materials must be washed in BSS (balanced salt solution) aseptically to avoid contamination. The tissue to be cultured should be properly sterilised with 70% ethanol, and remove surgically under aseptic conditions. Thus the tissue isolated should be either stored […]
6 Methods of DNA Sequencing | Biotechnology
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The following are the common methods of DNA sequencing:- 1. Sequencing 2. Manual Sequencing 3. Automated Sequencing 4. Southern Blotting 5. Northern Blotting 6. Western Blotting. Method # 1. Sequencing: One of the major methods of DNA sequencing in known as chain termination sequencing, dideoxy sequencing, or Sanger sequencing after its inventor biochemist Frederick Sanger. […]