Upload NowIn this article we will discuss about the principle, requirements and procedure for measuring the growth of bacteria by spectrophotometric method. Principle: This method relates cell number to turbidity of a broth culture. Turbidity or optical density (OD) is directly proportional to cell concentration. Requirements: 1. 10-12 hour E. coli broth culture maintained at lag […]
Measuring the Growth of Bacteria | Microbial Biotechnology
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Litmus Milk Reactions: Requirements and Procedure | Bacteria
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After reading this article you will learn about the requirements and procedure of litmus milk reactions. Milk sugar lactose, the milk proteins casein, lacto-albumin and lacto-globulin are the milk substrates that can be transformed by microorganisms, bringing about changes in pH. In order to distinguish the metabolic changes, a pH indicator, the oxidation reduction indicator […]
Actinomycetes: Isolation and Identification | Microbial Biotechnology
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In this article we will discuss about the isolation and identification of actinomycetes. Isolation of Actinomycetes: This group of prokaryotes are ubiquitious and are found in all ecosystems. Some genera like Actinomyces are anaerobic and others like Frankia (symbiotic) require specialised growth media and incubation condition. To isolate them from soil, special treatments are essential. […]