Upload Now
Read this article to learn about Agricultural Biotechnology in Developing Countries: Application to Plant Health, Genetic Improvement and Bio-diversity Based Biotechnology
Application to Plant Health:
Monoclonal antibodies, recombinant antigens, and molecular plant disease tests have greatly improved diagnostic systems and pathogen characterisation. The most widely used technique for generating pathogen/virus free plants in the region involved the combination of thermotherapy and meristem tip culture.
This tissue-culture-based application is still actively being used in most seed-production programmes of the region. The CIP and CIAT pioneered the development of these techniques for generating and distributing healthy plant stocks of vegetatively propagated crops (e.g., potato, sweetpotato, and cassava).
National programmes and private groups of Brazil, Chile, Uruguay, Cuba, Mexico, Argentina, Colombia, and Peru have developed and commercialised these technologies, to variable extent, for the production of healthy seed.
Tissue-culture-based propagation techniques have been used in the region over many years for the multiplication of planting material of many economically important crop plants, such as roots and tubers, flowers, fruit trees, and others. These propagation techniques are now being standardised for a range of native plants of commercial value, such as forests and ornamentals.
Bioreactors and temporary immersion systems are now available for industrial plant multiplication; however, development of this technology is still incipient in the region.
Important applications have been demonstrated for coffee somatic embryo multiplication at CATIE, for cassava multiplication, rice anther culture, and regeneration of genetically transformed cassava at CIAT, and for the regeneration and multiplication of potato clones at CIP.
Genetic Improvement:
For many LAC biotechnology groups, anther culture for haploid plant production and embryo rescue for hybrid plant production have been connected to crop improvement programmes, such as those in Mexico, Colombia, Costa Rica, Uruguay, Chile, and Brazil for rice and grain legumes. Molecular marker-assisted selection has improved plant breeding through the use of tightly linked markers in assisted selection.
Practical examples exist in the region for maize and wheat at CIMMYT; cassava, rice, and common bean at CIAT; potato and sweetpotato at CIP; and in the national programmes of Brazil, Mexico, Uruguay, Chile, Colombia, and Costa Rica. The LAC region has conducted 27% of the world’s field trials of 24 transgenic crops.
Argentina alone represents 21% and is second in the world in growing transgenic crops commercially; this has represented $200 million of profits to Argentina in 2000. Argentina, Brazil, Colombia, Honduras, Mexico, and Uruguay commercialise genetically engineered (GE) crops.
Brazil officially approved GE crops for planting in 2003 and has already become the fourth in the world in growing GE crops. Colombia approved 10,000 hectares of commercial Bt cotton and carnations for export. Bolivia has carried out field trials for GE cotton and soybeans, and Honduras is growing GE maize for the first time.
In Mexico, the Savia firm has performed within the ten top worldwide. Because the global market for transgenic crops is projected to reach $25 billion in 2010, this application area has generated large expectations in LAC countries. Biosafety and IPR regulations still have to be enforced in many countries for an effective and safe use of genetically engineered crops, especially if their production is meant for the export market.
The agricultural systems that prevail in significant sectors of the LAC region are characterised by a mosaic of continuous cropping systems, complex crop/pest management systems, and biological, cultural, and socioeconomic diversity, all of which need to be considered in the adoption strategies of any agricultural new technology, including genetically engineered crops.
Currently, the most commercialised biotechnological product in the LAC region has been transgenic seed, but it has been concentrated basically in one country. The next in importance is the selling of virus-free stocks and seeds, and in third place are biopesticides and other agricultural bioinputs. Countries which have used modern biotechnologies like genetic engineering have had a higher value of their seed market than those using mature technologies like tissue culture.
The FAO’s 2000 report pointed out to a general weakness in the quantity and quality of regional biotechnology researchers: Only 40% of the limited number available was postgraduates, and only about 10% had doctoral degrees. Most researchers were biologists or agronomists, with very few specialised in key areas such as molecular genetics, molecular biology, protein engineering, molecular and industrial microbiology, or bioinformatics.
An aggressive programme of personnel development needs to be implemented in most LAC countries in basic sciences and cutting-edge biotechnological applications in particular, in the Central American and the Andean regions-otherwise any biotechnological programme aimed at generating income and employment would face serious drawbacks.
By 1999, the LAC region invested only 0.59% of the IGP in research and development, with Brazil, Chile, and Cuba showing the highest level. However, even these lagged behind countries like Canada (1.61%), France (2.18%), the United States (2.84%), and Japan (3.06%). Dellacha et al. reported that 62% of the scientific and technological activities in LAC were funded through the state’s national science and technology councils, 28% by companies, and 9% by universities; funding coming from external sources was approximately 1%.
State support and private investment for biotechnology research varies in the region. In some countries, it is minimal; for example, in Peru the 1998 funds from the state amounted only to 0.06% of the IGP, while in Brazil state funding was 0.86% in 1999.
Biodiversity-based Biotechnology in Developing Countries:
The LAC region concentrates major biodiversity hotspots of the world. The region is also a center of origin and diversity of a number of species that sustain current world food supply (e.g., potato, sweetpotato, corn, tomato, beans, cassava, peanuts, pineapple, cacao, chili pepper, and papaya).
Furthermore, the greatest number of flowering plants with unusual sources of compounds for food and agriculture, as well as for the biopharmaceutical, nutraceutical, cosmetic, and environmental industries, exists in this region. This comparative advantage needs to be explored and sustainably utilised by integrating the emerging biotechnologies with the region’s rich traditional knowledge on the properties and attributes of its biological resources.
The world market for biological resource-derived ingredients and molecules in 1999 was US$925 billion for different sectors and industries. Several LAC research groups have begun to work with native forest and medicinal species with the view to explore commercial opportunities in the use of natural ingredients.
Application of simple bioprocessing technologies offers a short-term approach for the production of sweeteners, flavour products, fruit juices, amino acids, pigments, vitamins, and antioxidants. For example, the Agroindustrial Group Backus S.A. in Peru has used camu-camu, an Amazonian fruit of the Myrtaceae family, for ascorbic acid extraction.
The nutraceutical and biopharmaceutical products have become the third and fourth most important markets for biological resource-derived products. In order to tackle these emergent markets, new scientific specialisations are being created in universities and industry worldwide. Some have resulted from the merging of former branches of science; for example, ethno-botany has joined with pharmacology to form ethnopharmacology.
On the other hand, new tools are being developed for the automatisation and miniaturisation of genetic and chemical analyses and syntheses (e.g., robotics, DNA chips, combinatorial chemistry, liquid chromatography, mass spectrometry, X-ray spectrophotometry, nuclear magnetic resonance, among others).
The so-called new “omic” sciences (functional genomics, proteomics, and metabolomics) and bioinformatics are improving the search of chemical principles from plant species to develop new target-specific drugs. Not only are specialised personnel and modern equipment needed to achieve scale-up capabilities in this field, but appropriate business management, including IPR, are major factors that still limit commercial takeoff in the LAC region.
Since 1997, Brazil has implemented several genome projects in universities and private research laboratories under the management of a virtual institute, the Organisation for Nucleotide Sequencing and Analysis. The organisms selected include bacteria and plants important to Brazil’s economy and also of global interest (e.g., sugarcane, Xylella fastidiosa, Xanthomonas species, and Eucalyptus).
These studies involve high-throughput sequencing, functional genomics, proteomics, and transcriptomics, among other advanced technologies. The initial goal was the sequencing of the plant pathogen Xylella fastidiosa, which was achieved for the first time worldwide.
Other efforts in the region are in Mexico, Cuba, and recently in Chile, where the national genome project will tackle the functional genomics of grapevine and peach. A Latin American Genome Biology Network, sponsored by the United Nations University for Latin America and the Caribbean (UNUBIOLAC), has been created with the objective of enhancing linkages among Latin American groups working with high-throughput genomic technologies.
In 2003, a study in Andean countries (Bolivia, Colombia, Ecuador, Peru, and Venezuela) supported by CAF (Corporacion Andina de Fomento) and CEPAL (Economic Commission for Latin America and the Caribbean) was initiated with the goal of analysing biotechnological, institutional, and human-resource capacities involved in biotechnology research and development for biodiversity utilisation and generation of biotechnological products and biotrades.
The study also analyses the global/regional market trends for their potential commercialisation and eventually will offer recommendations and strategic guidelines in support of these countries to promote sustainable value-added transformation and commercialisation of products derived from biodiversity and biotechnology.
The regional CGIAR centres, the CIP, CIAT, and CIMMYT, and the regional organisation (CATIE) have played active roles for many years in the regional development of biotechnology applications to selected crop plants through the diffusion of advanced strategic technologies (including genomic), have offered technical and scientific training, and have developed collaborative work with national research institutions. REDBIO has had, and continues to have, a critical role in developing biotechnology science and applications in the LAC region.
We believe it is now time to turn attention to biodiversity-based biotechnology research and development. Another promising future role of biotechnology such as nitrogen fixation, photosynthetic efficiency, soil nutrient cycling efficiency, abiotic (drought, acidity, salinity) stress adaptation, and the reduction of off-farm inputs such as agrochemicals.
All have far- reaching implications for sustainable agricultural systems in LAC agroecologies like the Andean region. At present, however, most commercial biological products used in countries like Colombia and Cuba have been elaborated using the bacterium B. thuringiensis, the fungi Beauveria, Metarhizium, and Paecilomyces, and baculoviruses.
In recent years, private investment in agricultural R&D has increased, particularly in countries where effective and transparent regulatory frameworks are in place and comparative research infrastructure and qualified human resources exist.
A dynamic biotechnological industry, with clusters of companies and public research institutions, has begun to emerge in some LAC countries. Joint public/private ventures have explored collaborations to address bio-product market demands. One example is a bio-prospecting collaboration, under an ICBG project, between the Aguaruna community of Peru, three universities, and the G.D.
Searle Corporate Partnership, to apply ethnomedicinal approaches for examining plant biodiversity based on the Aguaruna pharmacopeia and involving a wide range of human diseases and syndromes. Patent applications have been filed as a result; benefit sharing with the communities has been included.
Another ICBG project in Panama is searching new bioactive compounds against cancer and the tropical parasites causing malaria, leishmaniasis, and Chagas’ disease, through a collaboration between Smithsonian Tropical Research Institute, several universities in Panama and one in the United States, and Novartis.
In Cuba, the Biotechnological West Pole of La Havana comprises more than 40 specialised biotechnology centres; others include the Bio Trade initiative for the promotion of biodiversity-derived products and services in Colombia, under the leadership of the Alexander von Humboldt Institute, and the emerging companies in Amazonia supported by UNCTAD-Bolsa Amazonica-IavH. The adoption of intellectual property and biosafety regulations has recently been promoted, but management and enforcement varies among LAC countries.
Chile is the only country where biotechnological processes can be patented. Microorganisms can be patented in Brazil and Mexico, but neither microorganisms nor genes can be patented in the Andean countries. In most countries, UPOV-type plant variety protection systems exist.
Stimulation of investments and facilitation of the acquisition of technologies through collaborative partnerships should go hand-in-hand with mechanisms to link the research with the holders of biological resources. Governments can offer tax and other incentives to investors; these incentives should encourage the sharing of the derived benefits with the research partners and with the traditional curators of genetic resources.
Finally, it is relevant to mention that food insecurity in large sectors of the LAC region is a consequence of inadequate social, economic, and technological development. The new biotechnologies open a range of opportunities for increasing biodiversity-based product diversification.
It is necessary to bear in mind not only the local socioeconomic context, but also the level of export/import of agricultural and biodiversity derived products; the importance of the small, medium, and large agro-industry in the economy; the country’s research and technological capacities; and the existence of a legal framework that stimulates biodiversity conservation and utilisation.
The situation of the resource-poor farmer should be taken into account, to make sure that the benefits of modern biotechnologies reach this important sector-currently a majority in some LAC countries.